Microplastic in the Black Sea
Material, methods, samples

Material Poza

  • glass containers for collecting sand from the area of breaking waves, water and sand on the beach
  • kits the analysis of physico-chemical factors
  • Petri dishes, glass slides
  • optical microscope (10X eyepiece, 10X lens, 20X, 40X, 18mm eyepiece lens diameter)
  • stereomicroscope (10X eyepiece, objective, 2X, 4X,, eyepiece lens diameter 22 mm)
  • metal sieves with a diameter of 2 mm, 0.5 μm, filter paper
  • graduated cylinder, pipettes, Berzelius glasses
  • tweezers, spatulate needle

Method for water samples Poza

  • 100 mL water samples are collected
  • is filtered using a 0.2 mm filter
  • is treated with 10% KOH solution and 30% H2O2 1:1
  • keep the mixture for 24 hours
  • isolate the microplastic from the supernatant
  • Transfer to a Petri dish
  • dry for 48 hours
  • observe under a stereomicroscope (magnification power of 20X and 40X) and optical microscope (magnification power of 100X, 200X or 400X)
  • take pictures
  • fill in the observation sheets

Method for sediment samples Poza

  • samples of 100 g of sediment are collected (from the water line and on transect lines on the beach)
  • transfer to 400 mL Berzelius beakers and treat with 28 Na NaCl solution to increase the density of the solution. Shake the solution
  • isolate the microplastic from the supernatant treated with 15% HCl to destroy the remaining organic matter
  • Transfer to a Petri dish
  • dry for 48 hours
  • observe under a stereomicroscope (magnification power of 20X and 40X) and optical microscope (magnification power of 100X, 200X or 400X)
  • take pictures
  • fill in the observation sheets

Method for bivalve samples

  • 50 mussels (Mytilus galloprovincialis) are collected
  • the digestive structures are dissected, isolated, mixed with a blender, then treated with 10% KOH solution and 30% H2O2 1:1 to remove organic matter
  • the solution is filtered and transfered to a Petri dish
  • dry for 48 hours
  • observed under a stereomicroscope (magnification power of 2X and 4X) and optical microscope (magnification power of 100X, 200X or 400X)
  • take pictures
  • fill in the observation sheets